วารสารวิทยาลัยบัณฑิตเอเซีย ...

Platelet-rich fibrin (PRF) serves as a source of growth factors for promoting tissue repair and regeneration. It has been used widely in dentistry. Heat-compression technique was developed for modifying the cross-linking of fibrin structure to reduce rate of degradation, but very few studies have been carried out until now. This study aimed to evaluate the effect of heat-compression on the level of transforming growth factor-beta1 (TGF-beta1), representative growth factor with high concentration in platelet-rich fibrin and key factor in wound healing process. PRF membranes were prepared from blood samples of ten healthy volunteers. Then the membrane samples were heat-compressed with different temperature and divided into six groups: Control (no heat), 60, 70, 80, 90 and 100 degrees Celsius. Extraction of growth factors were done immediately. TGF-beta1 concentration was measured with enzyme-linked immunosorbent assay (ELISA). Blood samples were collected from the same three volunteers for analysis of TGF-beta1 release. PRF membranes were placed in media (DMEM) for 14 days. TGF-beta1 release amounts were measure from collected media at five time periods. In immediate extraction part, heat compression significantly decreased the TGF-beta1level in comparison with that of control group. Moreover, the result showed a decrease TGF-beta1 level by increase temperature using for heat compression. The dramatic decrease of TGF-beta1 level was found in 90 and 100 degrees Celsius group. A high release of TGF-beta1 in the early study time was observed, follow by a marked decrease in the late study time with slight difference of TGF-beta1 release kinetics in each sample group.