This study aimed to quantify the collagen content and evaluate the anti-tyrosinase activity of extracts from the petals of Nelumbo nucifera Gaertn using the hydroxyproline assay for collagen quantification and the dopachrome method for assessing anti-tyrosinase activity. The findings indicated that the petal extract of Nelumbo nucifera Gaertn, at a concentration of 200 µg/mL, contained 33.94±2.85 µg of collagen/mL, with a positive correlation observed between higher extract concentrations and increased collagen content. Additionally, the anti-tyrosinase activity of the extract, measured at 200 µg/mL, demonstrated an inhibitory effect of 52.34±0.13 µg/mL, with a similar trend of enhanced inhibition at higher concentrations. These results suggest that the petal extract of Nelumbo nucifera Gaertn possesses significant collagen content and anti-tyrosinase activity, potentially contributing to reduced melanin synthesis and improved skin nourishment. Consequently, this extract holds promise for development into cosmeceutical products aimed at skin brightening and melanin reduction. Furthermore, the data provided can inform consumer choices and enhance the value of Thai herbal products.