The purpose of this research was to study factors associated with depression of nursing students in the online teaching situations of private nursing education institution in KhonKaen Province in 1st - 4th year. The sample through stratified random sampling, a total of 226. Data were collected using information by questionnaires into 4 part: 1) general information; 2) self-esteem questionnaire; 3) SuanPrung stress test 20 points; and 4) depression screening 9Q. Data were analyzed using descriptive statistics, Pearson correlation analysis and Chi-square test. The results found that 15.04% of nursing students had depression. The 1st year students had the most depression at 24.60%, followed by the 4th year, 3rd year and 2nd year students with 15.50%, 11.90%, and 9.10% respectively. Self-esteem was at a high level ("X" ̅=3.49, SD=0.52), with the 1st year students having the highest self-esteem average score ("X" ̅=3.58, SD = 0.54), followed by 4th, 3rd and 2nd year students, ("X" ̅= 3.48, SD = 0.52; "X" ̅= 3.44, SD = 0.51 and "X" ̅= 3.46, SD = 0.52 respectively). Nursing students had the most moderate levels of stress at 49.12 %, followed by a high level of stress at 28.32 %. The 1st year students were found to the most high levels of stress (47.30%), The 2st year students were found to a moderate levels of stress (58.00 %). The 3st year students were found to a moderate levels of stress (55.90%) and The 4st year students were found to have a moderate levels of stress (52.10%). The result from correlation analysis revealed a negative relation between the importance of self-esteem with depression (r =.218, p= .001). On the other hand, a positive relation was high levels of stress and severe stress with depression (r = .163, p = .015; r = .321, p = 0.01 respectively). The suggestion of this research should have activities to promote self-esteem and develop stress management skills to support the adaptation and prevention of depression among nursing students in online teaching situations.