The purpose of this experimental research was to study effect of using curriculum on a prevention course of liver fluke and bile duct cancer in school for upper secondary student level in the northeast region, Thailand in terms of knowledge, attributes, process skills and health behaviors. The sample group included upper secondary school in northeastern region consisted of 51 schools, 17 schools divided into experimental group and 2 control groups, in 17 schools each group. Research tools are a prevention Course of Liver Fluke and Bile Duct Cancer a high school in school for upper secondary student Level in the northeast region, Thailand and the curriculum effectiveness measure form. The data were analyzed by compare the average scores on immunity for liver fluke and bile duct cancer, consisting of: before and after using paired t-test statistics and comparing the average scores before and after of using the prevention course for liver fluke and bile duct cancer, between 3 groups was the experimental group and 2 control groups were analyzed by ANOVA statistics. The results showed that; average knowledge score after using the curriculum of all 3 groups, it was found that all 3 groups differed (p-value=.000), average rating for attributes after the course using was higher than before the course (p-value =.000), the mean score on process skills between the 3 groups after the course was different, and the students in all groups had healthy behavioral immunity (H1) was higher before using a course, and the experimental group had a greater proportion of healthy (H2) than the control group at the statistical significance level of 0.05.